Journal: Journal of Inflammation (London, England)

Article Title: Montelukast suppresses NLRP3 inflammasome activation as a potential prophylactic agent against gout arthritis flares

doi: 10.1186/s12950-026-00487-9

Figure Lengend Snippet: Montelukast attenuates inflammation related to GA flares in mice. The neutrophil marker (MPO) ( A ), inflammasome markers (IL-1β, IL-18) ( B, C ) and other pro-inflammatory cytokines/chemokines (CXCL1, IL-6, TNF-α) ( D-F ) affiliated with GA flares were determined in mouse paw homogenates (100 mg/ml) on day one post-MSU administration by ELISA. **** p < 0.0001, *** p < 0.001, * p < 0.05 using a Student’s t-test. Data for all graphs is presented as mean ± SEM

Article Snippet: WT BMDM supernatants from the montelukast inflammation assays were evaluated for IL-6 using the Mouse IL-6 DuoSet ELISA (R&D Systems, Cat. No. DY406), TNF-α using the Mouse TNF-α DuoSet ELISA (R&D Systems, Cat. No. DY410), and CXCL1 using the Mouse CXCL1/KC DuoSet ELISA (R&D Systems, Cat. No. DY453) following manufacturer’s instructions.

Techniques: Marker, Enzyme-linked Immunosorbent Assay

Journal: Clinical & Translational Immunology

Article Title: Immune activation and response dynamics of human iPSC ‐derived macrophages in tuberculosis infection models

doi: 10.1002/cti2.70071

Figure Lengend Snippet: iMacs demonstrate enhanced phagocytosis and cytokine production upon BCG infection. Microscopic analysis of immunofluorescent images stained for (a) vATPase and (b) LAMP‐1 immunolabelling after infection with BCG (MOI 10:1) for various time points, using a Leica TCS SP5 confocal microscope (100× oil objective, scale bar = 10 μm). Right panels (a) + (b) show Pearson correlation coefficient demonstrating correlation between (a) BCG (green) and v‐APTase (red) accumulation and (b) BCG (green) and LAMP‐1 (red) accumulation at different time points post‐infection. The data are representative of n = 3 independent experiments and individual cells were quantified according to the program. (c) IL‐6, (d) TNF and (e) IL‐1β secretion by iMacs and MDMs 6 h and 24 h post BCG infection with an MOI of 10:1, compared to non‐infected cells as controls measured by ELISA. Data (c) – (e) are represented as the mean ± SD of n = 4–5 biological replicates measured in duplicates. Statistical significance was determined using Mann–Whitney U test (* indicates P < 0.0332, ** indicates P < 0.0021); n.i., non‐infected control cells.

Article Snippet: ELISAs were performed using the Human IL‐6 DuoSet ELISA (R&D Systems, Cat. No. DY206) and the TNF‐alpha DuoSet ELISA (R&D Systems, Cat. No. DY210), following the manufacturer's instructions.

Techniques: Infection, Staining, Microscopy, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Control

Journal: Clinical & Translational Immunology

Article Title: Immune activation and response dynamics of human iPSC ‐derived macrophages in tuberculosis infection models

doi: 10.1002/cti2.70071

Figure Lengend Snippet: iMacs exhibit a beneficial immune response against HKMT . (a) Representative phase‐contrast microscopy images of Selene iMacs (left), NIH iMacs (middle) and MDMs (right) non‐stimulated or post‐HKMT stimulation using a Leica TCS SP5 confocal microscope (scale bars = 100 μm top and 200 μm bottom) ( n = 2). (b) Flow cytometric quantification of the percentage of phagocytic macrophages at different time points post‐HKMT stimulation ( n = 8–13). (c) ELISA quantification of IL‐6 and (d) TNF secretion 24 h post HKMT stimulation compared with non‐stimulated cells as controls ( n = 4–20). (e) Cytokine bead assay analysis of additional pro‐ and anti‐inflammatory cytokines 24 h post HKMT stimulation ( n = 3–6). (f) Flow cytometric quantification of ROS production upon HKMT stimulation. Statistical significance is indicated using a two‐tailored t ‐test ( n = 9–13). (g) RT‐qPCR analysis of Bax expression, (h) Cathepsin B expression and (i) LC3B expression in iMacs and MDMs post‐HKMT stimulation, presented as fold change relative to non‐infected cells as control ( n = 3–6). All data are presented as mean ± SD. All statistical significance was determined using a Mann–Whitney U test (* indicates P < 0.0332, ** indicates P < 0.0021, *** indicates P < 0.0002 and **** indicates P < 0.0001). n.s., non‐stimulated control cells.

Article Snippet: ELISAs were performed using the Human IL‐6 DuoSet ELISA (R&D Systems, Cat. No. DY206) and the TNF‐alpha DuoSet ELISA (R&D Systems, Cat. No. DY210), following the manufacturer's instructions.

Techniques: Microscopy, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Infection, Control, MANN-WHITNEY